INTERACTION OF ANTIGEN AND ANTIBODY ON CORE-SHELL POLYMERIC MICROSPHERES

+ 作者地址


0
  • 摘要
  • 参考文献
  • 相关文章
  • 统计
Monodispersed microspheres with polystyrene as the core and poly(acrylamide-co-N-acryloxysuccinimide) as the shell were synthesized by a two-step surfactant-free emulsion copolymerizatiun. The core-shell morphology of the microspheres was shown by scanning electron microscopy and transmission electron microscopy. Rabbit immunoglobulin G(as antigen) was covalently coupled onto the microspheres by the reaction between succinimide-activated ester groups on the shell of the microspheres and amino groups of the antigen molecules. The size of particles was characterized by dynamic light scattering technique and was found to vary upon bioconjugation and interaction with proteins. The binding process was shown to be specific to goat anti-rabbit immunoglobulin G (as antibody) and reversible upon the addition of free antigen into the system.

[1] Kamei,S;Okubo,M;Matsumoto,T .[J].Journal of Applied Polymer Science,1987,34:1439.

[2] Kondo,A;Kaneko,T;Higashitani,K .[J].Biotechnology and Bioengineering,1994,44:1.

[3] Delair,T;Marguet,V;Pichot,C;Mandrand,B .[J].Colloid and Polymer Science,1994,272:962.

[4] Miksa B.;Slomkowski S. .POLYPYRROLE CORE POLYACROLEIN SHELL LATEX FOR PROTEIN IMMOBILIZATION[J].Colloid and polymer science,1995(1):47-52.

[5] Yasui,M;Shiroya,T;Fujimoto,K;Kawaguchi,H .[J].Colloids and Surfaces B:Biointerfaces,1997,8:311.

[6] Chen JP;Su DR .Latex particles with thermo-flocculation and magnetic properties forimmobilization of alpha-chymotrypsin[J].Biotechnology Progress,2001(2):369-375.

[7] Horak D.;Karpisek M.;Turkova J.;Benes M. .Hydrazide-functionalized poly(2-hydroxyethyl methacrylate) microspheresfor immobilization of horseradish peroxidase[J].Biotechnology Progress,1999(2):208-215.

[8] Bastos-Gonzalez,D;Ortega-Vinuesa,J.L;de las Nieves,F.J;Hidalgo-Alvarez,R .[J].Journal of Colloid and Interface Science,1995,176:232.

[9] Margel,S;J.Polym.Sci .[J].Journal of Polymer Science Part A: Polymer Chemistry,1984,22:3521.

[10] Peula JM.;Nieves FJD.;Hidalgo-Alvarez R. .Covalent binding of proteins to acetal-functionalized latexes. I. Physics and chemical adsorption and electrokinetic characterization[J].Journal of Colloid and Interface Science,1998(2):132-138.

[11] Shimizu,N;Sugimoto,K;Tang,J;Nishi,T;Sato,I;Hiramoto,M;Aizawa,S;Hatakeyama,M;Ohba,R;Hatori,H;Yoshikawa,T;Suzuki,F;Oomori,A;Tanaka,H;Kawaguchi,H;Watanabe,H;Handa,H .High-performance affinity beads for identifying drug receptors.[J].Nature biotechnology,2000(8):877-881.

[12] Tomohiro,T;Sawada,J;Sawa,C;Nakura,H Yoshida,S Kodaka,M Hatakeyama,M Kawaguchi,H Handa,H.and Okumo,H .[J].Bioconjugate Chemistry,2002,13:163.

[13] Du,Y.Z;Tomohiro,T;Zhang,G;Nakamura,K.and Kodaka,M .[J].Chemical Communications,2004,5:616.

[14] Du YZ;Kodaka M .Preparation and characterization of biotinylated and enzyme-immobilized heterobifunctional latex particles as nanobio devices[J].Journal of Polymer Science, Part A. Polymer Chemistry,2005(3):562-574.

[15] Chen,G;Hoffman,A.S .[J].Bioconjugate Chemistry,1993,4:509.

[16] Ding,Z;Chen,G;Hoffman,A.S .[J].Bioconjugate Chemistry,1996,7:121.

[17] Malmstadt,N;Hyre,D.E;Ding,Z;Hoffman,A.S.and Stayton,P.S .[J].Bioconjugate Chemistry,2003,14:575.

[18] Takei,Y.G;Aoki,T;Sanui,K;Ogata,N Okano,T.and Sakurai,Y .[J].Bioconjugate Chemistry,1993,4:42.

[19] Takei,Y.G;Matsukata,M;Aoki,T;Sanui,K Ogata,N Kikuchi,N Sakurai,Y.and Okano,T .[J].Bioconjugate Chemistry,1994,5:577.

[20] Pollak,A;Blumenfeld,H;Wax,M;Baughn,R.L.and Whitesides,G.M .[J].Journal of the American Chemical Society,1980,102:102.

[21] Sun YM;Chen JP;Chu DH .Preparation and characterization of alpha-amylase-immobilized thermal-responsive composite hydrogel membranes.[J].Journal of biomedical materials research, Part B. Applied biomaterials,1999(2):125-132.

[22] Miyata,T;Asami,N;Uragami,T .[J].Macromolecules,1999,32:2082.

[23] Miyata,T;Asami,N;Uragami,T .[J].Nature,1999,399:766.

[24] Chen JP.;Chiu SH. .A poly(N-isopropylacrylamide-co-N-acryloxysuccinimide-co-2-hydroxyethyl methacrylate) composite hydrogel membrane for urease immobilization to enhance urea hydrolysis rate by temperature swing[J].Enzyme and Microbial Technology,2000(5/6):359-367.

[25] Meng QB;Li ZY;Li G;Zhu XX .Effect of hydrophilic shells on the formation of colloidal crystals by monodisperse core-shell polymer microspheres[J].Macromolecular rapid communications: Publishing the newsletters of the European Polymer Federation,2007(16):1613-1618.

[26] Chen YL;Gautrot JE;Zhu XX .Synthesis and characterization of core-shell microspheres with double thermosensitivity[J].Langmuir: The ACS Journal of Surfaces and Colloids,2007(26):1047-1051.

[27] Colonne,M;Chen,Y.L;Freiberg,S;Giasson,S.and Zhu,X.X .[J].Bioconjugate Chemistry,2007,18:999.

[28] Meng QB;Li ZY;Li G;Zhang X;An YL;Zhu XX .Aggregation of biotinylated polymeric microspheres induced by interaction with avidin[J].Pure and Applied Chemistry,2007(9):1575-1582.

[29] Li,F;Li,Z.Y;Guan,Y;Guo,L.L Li,X.Y Zhang,X.and Zhu,X.X.[J].Acta Polymerica Sinica,2008(07):673.

[30] Xiao,X.C;Chu,L.Y;Chen,W.M;Wang,S.and Li,Y .[J].Advanced Functional Materials,2003,13:847.

[31] Xiao,X.C;Chu,L.Y;Chen,W.M;Wang,S.and Xie,R .[J].Langmuir,2004,20:5247.

[32] Bradford,M.M .[J].Analytical Biochemistry,1976,72:248.


DOI: http://dx.doi.org/10.1007/s10118-010-1025-3

语种: 英文   

基金the National Natural Science Foundation of China(20328407;...


期刊热词
  • + 更多
  • 字体大小