猪囊尾蚴基质金属蛋白酶-9在猪肌肉和脑组织的表达分布

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1贵阳医学院医学检验系,贵州贵阳550004

2贵阳医学院寄生虫学教研室,贵州贵阳550004


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应用免疫组化分析猪囊尾蚴基质金属蛋白酶-9(MMP-9)的表达.用猪带绦虫卵1 mL(8万个/mL)灌胃20 d龄健康乳猪6头,于感染后40、80、120 d分别宰杀2头猪并取含囊尾蚴的肌肉及脑组织制作4μm厚石蜡切片,采用Envision二步法,观察不同时期寄生在肌肉及脑组织的猪囊尾蚴基质金属蛋白酶-9的表达.结果显示,不同时期寄生在肌肉及脑组织的猪囊尾蚴均表达MMP-9,随着感染时间增加,各组织猪囊尾蚴MMP-9的表达均变化不大(P>0.05),寄生在脑组织的猪囊尾蚴MMP-9的表达要略高于同期寄生在肌肉组织的猪囊尾蚴MMP-9的表达(P<0.05).感染部位组织学特征不同,猪囊尾蚴MMP-9的表达略有差异.

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DOI: http://dx.doi.org/10.3969/j.issn.1007-5038.2011.07.006

语种: 中文   

基金国察自然科学基金(30660168)

关键词猪囊尾蚴 基质金属蛋白酶-9 免疫组化


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