Genetic diversity analysis of Lepidium sativum (Chandrasur) using inter simple sequence repeat (ISSR) markers

Amandeep Kaur 已出版文章查询
Amandeep Kaur
1 Rakesh Kumar 已出版文章查询
Rakesh Kumar
1 Suman Rani 已出版文章查询
Suman Rani
1 Anita Grewal 已出版文章查询
Anita Grewal

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1Department of Biotechnology, University Institute of EngineeringTechnology, Kurukshetra University, Kurukshetra 136119, Haryana, India

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Lepidium sativum (commonly known as garden cress) belongs to the family Brassicaceae. It is a fast-growing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic, hypoglycemic, nephrocurative and nephroprotective activ-ities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat (ISSR) markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and repro-ducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were poly-morphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed. Cluster analysis based on unweighted pair-group method, arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L. sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range 32–72%genetic relatedness among the 18 genotypes.

[1] Acharya HS;Sharma V .Molecular characterization of opium poppy(Papaver somniferum)germplasm[J].Am J Infect Dis,2009,5:155-160.

[2] Bansal D;Bhasin P;Yadav OP;Punia A .Assessment of genetic diversity in Lepidium sativum(Chandrasur)a medicinal herb used in folklore remedies in India using RAPD[J].J Genet Eng Biotechnol,2012,10:39-45.

[3] Dharmar K;De-Britto AJ .RAPD analysis of genetic variability in wild populatins of Withania somnifera L.)dunal[J].Int J Biol Technol,2011,2:21-25.

[4] Farajpour, Mostafa;Ebrahimi, Mohsen;Amiri, Reza;Golzari, Rasoul;Sanjari, Sepideh.Assessment of genetic diversity in Achillea millefolium accessions from Iran using ISSR marker[J].Biochemical Systematics and Ecology,2012:73-79.

[5] Garcia-Vallve S.;Palau J.;Romeu A. .Horizontal gene transfer in glycosyl hydrolases inferred from codon usagein Escherichia coli and Bacillus subtilis[J].Molecular biology and evolution,1999(9):1125-1134.

[6] Juma AHA .The effects of Lepidium sativum seeds on fracture-induced healing in rabbits[J].Medscape General Medicine,2007,9(2):23.

[7] Korkovelos A.E.;Mavromatis A.G.;Huang W.G.;Hagidimitriou M.;Giakoundis A.;Goulas C.K. .Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species[J].Scientia horticulturae,2008(3):305-310.

[8] Kumar J;Mishra GP;Singh H;Srivastava RB Naik PK .Congruence of inter simple sequence repeats(ISSR)and random amplification of polymorphic deoxyribonucleic acid(RAPD)markers in genetic characterization of Artemisia annua in the Trans-Himalayan region[J].J Med Plants Res,2011,5(23):5568-5576.

[9] Li, Shan;Li, Jing;Yang, Xiao-Ling;Cheng, Zhou;Zhang, Wen-Ju . .Genetic diversity and differentiation of cultivated ginseng (Panax ginseng C. A. Meyer) populations in North-east China revealed by inter-simple sequence repeat (ISSR) markers[J].Genetic Resources and Crop Evolution,2011(6):815-824.

[10] Maier UH;Gundlach H;Zenk MH .Seven imidazole alkaloids from Lepidium sativum[J].PHYTOCHEMISTRY,1998,49(6):1791-1795.

[11] Mali RG;Mahajan SG;Mehta AA .Lepidium sativum(Garden cress):a review of contemporary literature and medicinal properties[J].Orient Pharm Exp Med,2007,7(4):331-335.

[12] Mukhopadhyay D;Parihar SS;Chauhan JS;Preeti Joshi SC .Effect of temperature and desiccation on seed viability of Lepidium sativum L[J].N Y Sci J,2010,3:34-36.

[13] Murray MG;Thompson WF .Rapid isolation of high molecular weight plant DNA[J].Nucleic Acids Research,1980,8:4321-4325.

[14] Ottai MES;Mostafa EAH;Ibrahim MM .Hereditary perfor-mance of three Lepidium sativum cultivars in Egypt[J].Aust J Basic Appl Sci,2012,6(3):169-175.

[15] Deepshikha Pande;Sapna Malik;Madhumati Bora;P. S. Srivastava .A rapid protocol for in vitro micropropagation of lepidium sativum linn. and enhancement in the yield of lepidine[J].In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association,2002(5):451-455.

[16] Paranjape AN;Mehta AA .A study on clinical efficacy of Lepidium sativum seeds in treatment of bronchial asthma[J].Iran J Pharmacol Ther,2006,5:55-59.

[17] Rahimmalek M;Sayed Tabatabaei BE;Arzani A;Etermadi N .Assessment of genetic diversity among and within Achillea species using AFLP[J].Biochemical Systematics and Ecology,2009,37:354-361.

[18] Saghai-Maroof MA;Soliman KM;Jorgensen RA;Allard RW .Ribosomal DNA spacer-length polymorphisms in barley.Men-delian inheritance,chromosomal location and population dya-namics[J].Proceedings of the National Academy of Sciences(USA),1984,81:8014-8018.

[19] Sarwat Maryam;Das S.;Srivastava P. S. .Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb[J].Plant Cell Reports,2008(3):519-528.

[20] Singh BK;Thakur AK;Rai PK .Genetic diversity and relationships in wild species of Brassica and allied genera as revealed by cross-transferable genomic STMS marker assays[J].Aust J Crop Sci,2012,6(5):815-821.

[21] Souframanien, J;Gopalakrishna, T .A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers[J].Theoretical and Applied Genetics,2004(8):1687-1693.

[22] Wang XM;Hou XQ;Zhang YQ;Yang R Feng SF Ren Y .Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by inter-simpe sequence repeat(ISSR)markers[J].INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13:3900-3915.

[23] Xu Q;Wen XP;Deng XX .A simple protocol for isolating genomic DNA from chestnut rose(Rosa roxburghiiTratt)for RFLP and PCR Analyses[J].Plant Molecular Biology Reporter,2004,22:301a-301g.

[24] Yadav YC;Srivastav DN;Seth AK;Saini V Yadav KS .Nephropharmacological activity of ethanolic extract Lepidium sativum L.Seeds in albino rats using cisplatin induced acute renal failure[J].Int J Pharm Sci Rev Res,2010,4(3):64-68.

[25] Fusheng ZHANG;Yali Lv;Hailing DONG;Shunxing GUO .Analysis of Genetic Stability through Intersimple Sequence Repeats Molecular Markers in Micropropagated Plantlets of Anoectochilus formosanus HAYATA, a Medicinal Plant[J].Biological & pharmaceutical bulletin,2010(3):384-388.

[26] Zietkiewicz E;Rafalski A;Labuda D .Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].GENOMICS,1994,20:176-183.


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