银杏叶制剂对大鼠脑缺血再灌注后N-甲基-D-天冬氨酸受体表达的影响及脑保护作用

李晓红 已出版文章查询
李晓红
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1 张峰 已出版文章查询
张峰
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1 赵明霞 已出版文章查询
赵明霞
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张秋玲
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3 赵忠新 已出版文章查询
赵忠新
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1山东大学附属济南市中心医院神经科,山东,济南,250013

2第二军医大学长征医院神经科,上海,200003

3泰山医学院生理学教研室,山东,泰安,271000


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目的研究局部脑缺血再灌注后N-甲基-D-天冬氨酸(NMDA)受体Ⅰ型亚单位(NR1)mRNA表达与细胞凋亡的关系及银杏叶制剂(Egb)对其影响.方法140只SD大鼠被随机分成假手术组、缺血再灌组,即MCAO组和Egb治疗组.每组大鼠再随机分成4组:分别在再灌注后6,24,48,96 h处死.进行TUNEL染色检测缺血区细胞凋亡情况,并用原位杂交方法检测NR1 mRNA.结果MCAO组大鼠大脑皮质梗死灶的凋亡细胞和NR1mRNA表达与假手术组比较明显增强(P<0.01),而Egb治疗组这两项指标的表达明显低于MCAO组(P<0.01).结论再灌注后脑组织中NR1mRNA表达增强,与细胞凋亡密切相关.银杏叶制剂可以明显抑制NR1mRNA的表达,减轻细胞凋亡.

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关键词脑缺血 NMDA受体 银杏叶制剂 凋亡 缺血再灌注


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