热应激环境下蛋鸡肠道微生物菌群多样性

李永洙 已出版文章查询
李永洙
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1 李进 已出版文章查询
李进
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2 张宁波 已出版文章查询
张宁波
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1 陈常秀 已出版文章查询
陈常秀
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1 CUI Yongquan 已出版文章查询
CUI Yongquan
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1临沂大学生命科学学院,临沂,276000

2山东龙盛农牧集团,临沂,276000

3国立冈山大学自然科学研究科,日本冈山700-8530


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通过分析热应激环境下蛋鸡肠道菌群结构的变化,旨在揭示热应激对肠道微生态环境的影响机理,为探索炎热环境下家禽肠道菌群定植规律提供理论依据.试验选择16周龄济宁百日鸡96只,随机分成对照组[(24±1)℃,Ⅰ]和热应激[(38±1)℃]组,分别在2个人工环境气候舱中饲养,各组设6个重复,每个重复8只,试验持续14 d.采用16S rDNA的变性梯度凝胶电泳(Denatured gradient gel electrophoresis,PCR-DGGE)技术和实时荧光定量(Real-time quantitative RT-PCR)以及多变量统计(Principal Components Analysis,PCA)分析等手段,分析热应激2d(Ⅱ)、7d(Ⅲ)和14 d(Ⅳ)时,对十二指肠、空肠及回肠内容物菌群多样性以及菌群数量变化.PCA分析结果显示,热应激暴露过程中十二指肠部位菌群组成与对照组保持相似的趋势,但热应激2d时空肠部位菌群组成有分开趋势;到7d时空肠与回肠部位菌群组成分开明显,而14 d时菌群组成具有明显差异;热应激7、14 d时空肠和回肠部位末检测到敏感乳杆菌(Lactobacillus agilis),回肠部位也末检测到约氏乳杆菌(Lactobacillus johnsonii)、不可培养细菌(Uncultured bacterium)等芽孢杆菌纲,而热应激不同时间段空肠和回肠部位可检测到不可培养细菌(Uncultured Escherichia sp)、溃疡拟杆菌(Bacteroides helcogenes)、卵形拟杆菌(Bacteroides ovatus)和索氏志贺氏菌(Shigella sonnei)等拟杆菌纲和γ-变形菌纲;其中约氏乳杆菌、敏感乳杆菌数量变化在空肠部位减少最明显(P<0.05),空肠和回肠卵形拟杆菌、不可培养的拟杆菌数量明显上升(P<0.05).热应激环境下蛋鸡空肠、回肠部位菌群多样性较为丰富,其抑制乳杆菌属、不可培养细菌的增殖,促进卵形拟杆菌的繁殖,而促进卵形拟杆菌的繁殖,导致消化道菌群平衡的破坏.

[1] Liu F;Yin J;Du M;Yan P;Xu J;Zhu X;Yu J .Heat-stress-induced damage to porcine small intestinal epithelium associated with downregulation of epithelial growth factor signaling[J].Journal of Animal Science,2009(6):1941-1949.

[2] Quinteiro-Filho, W. M.;Rodrigues, M. V.;Ribeiro, A.;Ferraz-de-Paula, V.;Pinheiro, M. L.;Sa, L. R. M.;Ferreira, A. J. P.;Palermo-Neto, J. .Acute heat stress impairs performance parameters and induces mild intestinal enteritis in broiler chickens: Role of acute hypothalamic-pituitary-adrenal axis activation[J].Journal of Animal Science,2012(6):1986-1994.

[3] Quinteiro-Filho, W. M.;Ribeiro, A.;Ferraz-de-Paula, V.;Pinheiro, M. L.;Sakai, M.;Sa, L. R. M.;Ferreira, A. J. P.;Palermo-Neto, J. . .Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens[J].Poultry Science,2010(9):1905-1914.

[4] Burkholder KM;Thompson KL;Einstein ME;Applegate TJ;Patterson JA .Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to salmonella enteritidis colonization in broilers.[J].Poultry Science,2008(9):1734-1741.

[5] Hansen J;Gulati A;Sartor RB .The role of mucosal immunity and host genetics in defining intestinal commensal bacteria.[J].Current opinion in gastroenterology,2010(6):564-571.

[6] Suliaman R H O;Malik H E E;Yousif I A .Effect of dietary protein level and strain on growth performance of heat stressed broiler chicks[J].International Journal of Poultry Science,2012,11(10):649-653.

[7] 李永洙,Yongquan Cui.利用PCR-DGGE方法分析不同鸡群的盲肠微生物菌群结构变化[J].生态学报,2011(21):6513-6521.

[8] 倪学勤,GONG Joshua,YU Hai,曾东,SHARIF Shayan,周小秋.采用PCR-DGGE技术分析蛋鸡肠道细菌种群结构及多样性[J].畜牧兽医学报,2008(07):955-961.

[9] 马俊孝,季明杰,孔健.PCR-DGGE技术在微生物物种多样性研究中的局限性及其解决措施[J].食品科学,2008(05):493-497.

[10] Cuív P (O);Klaassens E S;Smith W J;Mondot S,Durkin A S,Harkins D M,Foster L,McCorrison J,Torralba M,Nelson K E,Morrison M .Draft genome sequence of Enterococcus faecalis PC1.1,a candidate probiotic strain isolated from human feces[J].Genome Announcements,2013,1(01):e00160-e00112.

[11] Selim, ASM;Boonkumklao, P;Sone, T;Assavanig, A;Wada, M;Yokota, A .Development and assessment of a real-time PCR assay for rapid and sensitive detection of a novel thermotolerant bacterium, Lactobacillus thermotolerans, in chicken feces[J].Applied and Environmental Microbiology,2005(8):4214-4219.

[12] Sakai, M;Matsuka, A;Komura, T;Kanazawa, S .Application of a new PCR primer for terminal restriction fragment length polymorphism analysis of the bacterial communities in plant roots[J].Journal of Microbiological Methods,2004(1):81-89.

[13] Matsuka A.;Sakai M.;Kanazawa S. .Application of T-RFLP analysis for bacterial community structure of colonies grown on agar plates[J].Journal of the Faculty of Agriculture, Kyushu University,2003(1/2):107-112.

[14] Sakai JS;Kleckner N;Yang X;Guhathakurta A .Tn10 transpososome assembly involves a folded intermediate that must be unfolded for target capture and strand transfer.[J].EMBO Journal,2000(4):776-785.

[15] 白洁,李海艳,赵阳国.黄海北部不同站位海洋细菌群落分布特征[J].微生物学报,2009(03):343-350.

[16] Denman SE;McSweeney CS .Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen[J].FEMS Microbiology Ecology,2006(3):572-582.

[17] Walter J;Hertel C;Tannock GW;Lis CM;Munro K;Hammes WP .Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissellaspecies in human feces by using group-specific PCR primers and denaturinggradient gel electrophoresis[J].Applied and Environmental Microbiology,2001(6):2578-2585.

[18] Satokari RM;Vaughan EE;Akkermans ADL;Saarela M;de Vos WM .Bifidobacterial diversity in human feces detected by genus-specific PCRand denaturing gradient gel electrophoresis[J].Applied and Environmental Microbiology,2001(2):504-513.

[19] Huijsdens XW;Linskens RK;Mak M;Meuwissen SG;Vandenbroucke Grauls CM;Savelkoul PH .Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR.[J].Journal of Clinical Microbiology,2002(12):4423-4427.

[20] Isabel Taverniers;Erik Van Bockstaele;Marc De Loose .Cloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methods[J].Analytical and bioanalytical chemistry,2004(5):1198-1207.

[21] Taverniers I;Windels P;Vaitilingom M;Milcamps A;Van Bockstaele E;Van den Eede G;De Loose M .Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola[J].Journal of Agricultural and Food Chemistry,2005(8):3041-3052.

[22] Xiang Y. Zhu;Tanya Zhong;Yoga Pandya;Rolf D. Joerger .16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens[J].Applied and Environmental Microbiology,2002(1):124-137.

[23] Torok VA;Ophel-Keller K;Loo M;Hughes RJ .Application of methods for identifying broiler chicken gut bacterial species linked with increased energy metabolism[J].Applied and Environmental Microbiology,2008(3):783-791.

[24] Kretzschmar-Mccluskey V.;Curtis P. A.;Anderson K. E.;Kerth L. K.;Berry W. D. .Influence of hen age and molting treatments on shell egg exterior, interior, and contents microflora and Salmonella prevalence during a second production cycle.[J].Poultry Science,2008(10):2146-2151.

[25] 尹业师,王欣.影响实验小鼠肠道菌群的多因素比较研究[J].实验动物科学,2012(04):12-18.

[26] Rehman HU;Vahjen W;Awad WA;Zentek J .Indigenous bacteria and bacterial metabolic products in the gastrointestinal tract of broiler chickens[J].Archives of Animal Nutrition,2007(5):319-335.

[27] Ahir V B;Singh K M;Tripathi A K;Mathakiya R A Jakhesara S J Koringa P G Rank D N Jhala M K Joshi C G .Study of bacterial diversity in poultry gut using denaturing gradient gel electrophoresis[J].Iranian Journal of Applied Animal Science,2012,2(03):227-232.

[28] Kim, G. -B.;Seo, Y. M.;Kim, C. H.;Paik, I. K. . .Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers[J].Poultry Science,2011(1):75-82.

[29] 冯焱,杨小军,胡雄兵,刘烨,尹瑞卿,覃定奎,姚军虎.免疫应激对肉鸡肠道微生物区系的影响[J].农业生物技术学报,2012(07):807-814.

[30] Mountzouris KC;Tsirtsikos P;Kalamara E;Nitsch S;Schatzmayr G;Fegeros K .Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities[J].Poultry Science,2007(2):309-317.

[31] Bai, S. P.;Wu, A. M.;Ding, X. M.;Lei, Y.;Bai, J.;Zhang, K. Y.;Chio, J. S. .Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens.[J].Poultry Science,2013(3):663-670.

[32] Mathan M M;Mathan V I .Morphology of rectal mucosa of patients with shigellosis[J].Reviews of Infectious Diseases,1991,13(z4):S314-S318.

[33] 刁有祥,李久芹,陈庆普.山东省鸡大肠杆菌的分离鉴定[J].中国预防兽医学报,2002(01):21-23.

[34] Deng, W.;Dong, X. F.;Tong, J. M.;Zhang, Q. .The probiotic <i>Bacillus licheniformis</i> ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens.[J].Poultry Science,2012(3):575-582.

[35] James R. Chambers;Joshua Gong .The intestinal microbiota and its modulation for Salmonella control in chickens[J].Food Research International,2011(10):3149-3159.

[36] Jin LZ;Ho YW;Ali MA;Abdullah N;Jalaludin S .EFFECT OF ADHERENT LACTOBACILLUS SPP ON IN VITRO ADHERENCE OF SALMONELLAE TO THE INTESTINAL EPITHELIAL CELLS OF CHICKEN[J].Journal of Applied Bacteriology,1996(2):201-206.

[37] Swennen Q;Delezie E;Collin A;Decuypere E;Buyse J .Further Investigations on the Role of Diet-Induced Thermogenesis in the Regulation of Feed Intake in Chickens: Comparison of Age-Matched Broiler versus Layer Cockerels[J].Poultry Science,2007(5):895-903.


DOI: http://dx.doi.org/10.5846/stxb201305050933

语种: 中文   

基金国家高技术研究发展计划(2013AA102501)

关键词热应激 蛋鸡 肠道菌群 多样性


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