SeMNPV抑制HaSNPV诱导的Se-UCR细胞凋亡

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1中国科学院武汉病毒研究所,分子病毒学重点实验室,湖北武汉,430071


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在采用共感染和共转染的方法构建扩大杀虫范围的重组病毒的研究过程中发现棉铃虫单核衣壳核多角体病毒(Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus, HaSNPV)能诱导甜菜夜蛾细胞Se-UCR发生典型凋亡,但不能诱导另一株甜菜夜蛾细胞Se-301产生凋亡.以5 MOI的HaSNPV感染Se-UCR,在12h左右可以观测到少量细胞凋亡,24h能观察到明显的凋亡,凋亡细胞数量随时间不断增加,到72h基本上所有的细胞均发生凋亡,成为凋亡小体,基因组DNA片段化.同时发现HaSNPV诱导的甜菜夜蛾Se-UCR细胞凋亡能够被甜菜夜蛾多核衣壳核多角体病毒(Spodoptera exigua multicapsid nucleoplyhedrovirus, SeMNPV)所抑制, 进一步点杂交试验发现SeMNPV 和HaSNPV共同感染Se-UCR获得了HaSNPV在该细胞中的复制.

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语种: 英文   

基金中国科学院青年基金

关键词细胞凋亡 棉铃虫单核衣壳核多角体病毒 甜菜夜蛾多核衣壳核多角体病 Se-UCR细胞


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