斑节对虾亲环素A(cyclophilinA)基因的克隆及启动子序列分析

王卫芳 已出版文章查询
王卫芳
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1 邱丽华 已出版文章查询
邱丽华
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2 黄建华 已出版文章查询
黄建华
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2 张殿昌 已出版文章查询
张殿昌
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2 苏天风 已出版文章查询
苏天风
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2 杨其彬 已出版文章查询
杨其彬
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2 江世贵 已出版文章查询
江世贵
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1中国水产科学研究院,南海水产研究所,广东广州510300;华南农业大学,动物科学院,广东广州510642

2中国水产科学研究院,南海水产研究所,广东广州510300


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通过同源克隆和RACE技术获得斑节对虾(Penaeus monodon)亲环素A(cyclophilin A,CYPA)的cDNA序列.根据已克隆的cDNA序列设计引物,利用染色体步移技术(Cenomic DNA walking)从斑节对虾卵巢组织中克隆了CYPA基因的启动子和基因组DNA序列.测序结果表明,斑节对虾亲环素A(PmCYPA)cDNA全长834 bp,其中开放阅读框(open reading frame,ORF)为495 bp,可编码164个氨基酸;5'非编码区为31 bp,3'非编码区为308 bp.从斑节对虾基因组文库中扩增的CYPA基因组DNA全长3 181 bp,其中包括启动子区域1 173 bp,1个内含子426 bp,2个外显子序列:分别为101 bp和399bp.在5'UTR上游区域有明显的启动子序列,包含一个GC盒和2个CAAT盒,同时还包含AP1、CRE等调控元件,符合真核生物典型的启动子特征.分析基因的结构表明所克隆的PmCYPA符合PPlase家族成员特征,属于此基因家族成员.

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语种: 中文   

基金国家高技术研究发展计划(863计划)(2006AA603120)

关键词斑节对虾 cyclophilinA基因 克隆 启动子 序列分析


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