中国明对虾组织蛋白酶L的原核重组表达及其组织分布

卜兴江 已出版文章查询
卜兴江
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1 仉晓文 已出版文章查询
仉晓文
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2 孙允东 已出版文章查询
孙允东
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2 赵小凡 已出版文章查询
赵小凡
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2 王金星 已出版文章查询
王金星
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jxwang@sdu.edu.cn
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1山东大学,生命科学学院,山东,济南,250100;安徽师范大学,生命科学学院,安徽,芜湖,241000

2山东大学,生命科学学院,山东,济南,250100


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组织蛋白酶L属于半胱氨酸蛋白酶中的木瓜蛋白酶超家族,在哺乳动物中它与抗原提呈、肿瘤入侵和转移、骨质吸收、细胞凋亡等许多生命活动有关.但对其在无脊椎动物中的功能了解的不多.从中国明对虾(Fenneropenaeuschinensis)中克隆得到了组织蛋白酶L基因,为了进一步研究组织蛋白酶L的组织分布及其功能,对该基因进行了重组表达.将中国明对虾组织蛋白酶L基因的酶原片段亚克隆进原核表达载体pET30a中,转化大肠杆菌BL21(DE3),再进行诱导表达,表达产物经SDS-PAGE电泳检测,其分子量在40 kD左右,与期望的中国明对虾组织蛋白酶L的分子量一致.表达产物形成包涵体,经过对包涵体变性和复性,并进行His-tag柱亲和纯化,得到了纯化的蛋白.利用重组蛋白制备了的兔抗血清.Western实验表明,组织蛋白酶L在中国明对虾的肝、胃、肠中有表达,而在卵巢中没有表达.[中国水产科学,2008,15(6):910-916]

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语种: 中文   

基金国家自然科学基金(30770282)

关键词中国明对虾 组织蛋白酶L 原核重组表达 组织分布


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