海水养殖鱼哈维弧菌分离株的耐药谱型分析

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1中国水产科学研究院 南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东 广州 510300; 上海海洋大学 水产与生命学院,上海 201306

2中国水产科学研究院 南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东 广州 510300


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为了解和分析南海沿岸地区临床分离的哈维弧菌(Vibrio harveyi)菌株耐药谱多样性,采用K-B法对84株哈维弧菌分离株进行了12种抗生素的敏感性实验,并通过聚类分析对分离菌株耐药性进行研究,探讨其抗生素耐药性同菌株时空分布的关系。实验结果显示,84株哈维弧菌的耐药谱型数量为26种,谱型丰富度为31.0%多重耐药谱型数量(耐药种数3种以上)为13种,占总耐药谱型数量的50.0%,谱型丰富度达59.1%。海南、广东、广西、福建的分离株分别包含有18种、15种、3种、1种耐药谱型,4个地区均具有J型(FUR/AMO)耐药谱2007―2012年各年份菌株耐药谱型为2007年2种(J、M,耐2种抗生素)、2010年4种(A、B、J、M,耐5种抗生素)、2011年24种(除Y、Z外的A-X耐药谱型,耐7种抗生素),2012年26种(包括Y、Z耐药谱型,耐8种抗生素)。聚类分析将实验菌株分为ivi共6个亚群,进而聚类为Group I、Group II两个组群各亚群菌株具有独特的耐药谱型,耐药谱型分别是N、P、T、U、Y(i亚群), R、S(ii亚群), K、P(iii亚群), F、H、O、X、V、W(iv亚群), E、G、Q(v亚群), C、L、Z(vi 亚群)两个组群菌株没有共同的耐药谱型。研究结果表明,哈维弧菌分离株耐药谱型多样,谱型丰富度较高,来源不同的菌株耐药谱型存在差异,聚类分析方法能够根据耐药性情况有效地对菌株分型。

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DOI: http://dx.doi.org/10.3724/SP.J.1118.2015.00098

语种: 中文   

基金国家自然科学基金项目(31202027)

关键词哈维弧菌 海水养殖 耐药谱 聚类分析 亚群


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