ripply1 在斑马鱼早期胚胎背腹发育中的作用

孟亚平 已出版文章查询
孟亚平
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1 刘春业 已出版文章查询
刘春业
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2 石德利 已出版文章查询
石德利
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1山东大学生命科学学院,济南 250100;清华大学生命科学学院,北京 100084

2山东大学生命科学学院,济南,250100


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目的 探究ripply1基因在斑马鱼早期背腹轴发生过程中的作用. 方法 利用斑马鱼整封原位杂交技术揭示ripply1基因在斑马鱼早期胚胎发育过程中的表达模式,通过显微注射技术在胚胎1细胞期注射ripply1的mRNA来高表达Ripply1蛋白并在后期观察胚胎背腹标记基因的变化及胚胎形态变化. 利用Tol2转基因技术构建ripply1启动子驱动的GFP转基因鱼. 结果 原位杂交结果显示ripply1基因在斑马鱼原肠早期胚盾期特异表达在胚盾处,即预定的背部. 高表达ripply1后,在胚盾期背部标记基因表达范围扩大,腹部标记基因表达减弱,受精后24小时胚胎表现出严重的背部化表型:头部增大,腹部卵黄延伸减弱,尾部躯干及尾部区域减少,有的甚至形成了第二个体轴. 得到的转基因鱼揭示ripply1母源表达,并且转录起始位点上游1200个碱基驱动的GFP能模拟内源基因的表达图式. 结论 ripply1可能参与斑马鱼胚胎早期背腹轴的发生.

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DOI: http://dx.doi.org/10.3969/j.issn.1005-4847.2015.05.002

语种: 中文   

基金国家自然基金项目资助(31271556, 31471360, J1103515)

关键词斑马鱼 ripply1 早期胚胎发育 背腹发生


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