黄鳝EST-SSR标记的开发及其通用性检测

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陈志永
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1江西农业大学生物科学与工程学院, 江西南昌 330045

2江西农业大学动物科学技术学院, 江西南昌 330045


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利用SSRrepeat软件对NCBI数据库中黄鳝肠道EST序列进行挖掘、验证,分析微卫星(SSR)在这些序列中的总体分布特点,并开发黄鳝EST-SSR引物。结果显示,在黄鳝肠道的814条EST序列中共搜索到51个SSR位点,分布在43条EST中,出现频率为6.27%。这些EST-SSR的平均长度为18.80 bp。其中,三核苷酸重复为主导类型,占总SSR的49.02%,其次为二核苷酸重复,占29.41%。四、五、六碱基重复序列分别占微卫星总数的3.92%、11.76%、5.88%。多态性引物验证结果表明,在设计合成的21对EST-SSR引物中,12对在黄鳝中获得有效扩增。其中7对引物显示出多态性,共扩增出29个等位基因;每个位点等位基因数为2~7个(平均4.14),观测杂合度(
Ho<<)为0~0.857 1,期望杂合度(
He<<)为0.135 1~0.822 7,多态信息含量(
PIC<<)为0.123 8~0.781 1。在鲤形目和鲈形目中进行的跨物种扩增结果表明,12对引物中有7对引物在5个不同物种间得到成功扩增,5对引物在个别物种间表现出多态。研究获得的EST-SSR引物,可用于黄鳝的种资资源评价及遗传结构研究。

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DOI: http://dx.doi.org/10.13836/j.jjau.2017103

语种: 中文   

基金国家自然科学基金项目(31160530,31360634);江西省青年科学基金项目(20114BAB214003);...

关键词黄鳝 表达序列标签 微卫星 通用性


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